Syllabus For The Subject BIOTECHNOLOGY

UNIT - 01

BIOTECHNOLOGY - DEFINITION - CONCEPTS - HISTORY - ACHIEVEMENTS AND SCOPE -

PLANT TISSUE CULTURE AND POLLEN CULTURE - SOMATIC EMBRYOGENESIS - CALLUS

CULTURE AND SELECTION OF MUTANTS - DISEASE RESISTANT.

UNIT - 02

SALT TOLERANCE - DRAUGHT TOLERANCE - NUTRITIONAL QUALITY - HERBICIDE

TOLERANCE VARIETIES.

UNIT - 03

PLANT BIOTECHNOLOGY - PROTOPLAST CULTURE AND SOMATIC HYBRIDIZATION -

ISOLATION OF PROTOPLAST - CULTURE METHODS - FUSION METHODS - PRODUCTION

OF CYBRIDS AND HYBRIDS.

UNIT - 04

ENZYMES - RESTRICTION ENDO NUCLEASES - NOMENCLATURE - CLASSIFICATION AND

APPLICATION.

UNIT - 05

FUNCTION OF NUCLEASES - METHYLASES - LIGASES.

UNIT - 06

DEFINITION OF GENE - STRUCTURES - CLONING - SALIENT FEATURES - PLASMIDS -

COSMIDS - SINGLE STRANDED DNA VIRUSES - TI PLASMID - CONSTRUCTION OF

PLASMID VECTORS - LAMPDA PHAGES.

UNIT - 07

VECTORS - M13 VECTORS THEIR USES IN CLONING AND SEQUENCING - EXPRESSION

VECTORS GENOMIC LIBRARIES - C DNA LIBRARIES - GENE BANKS.

UNIT - 08

GENERAL CONSIDERATIONS OF MICROBIAL STRAIN IMPROVEMENT FOR

PHARMACEUTICAL PRODUCTS AND INDUSTRIES - GROWTH HORMONE AND

ANTIBIOTICS - GENE THERAPY METHODS.

UNIT - 09

STRAIN IMPROVEMENT FOR PRODUCTION OF AGRICULTURE PRODUCTS - SCP (ALGAE,

YEAST. MUSHROOM) - BIOFERTILIZERS - (BGA, VAM).

UNIT - 10

BIO PESTICIDES -BACILLUS THURINGIENSIS - FUEL - ETHONAL - MICROBIAL ALGAL

TECHNOLOGY.

UNIT - 11

TECHNIQUES EMPLOYED IN RECOMBINANT DNA - AGAROSE GEL ELECTROPHORESIS.

UNIT - 12

Vinayaka Missions University,Directorate of Distance Education

Salem India

BACHELOR OF SCIENCE IN BOTANY

3 Yr.

BIOTECHNOLOGY (BSC-BOT)(1030805)

PULSE GEL ELECTROPHORESIS - SOUTHERN - NORTHERN - WESTERN BLOTTING -

APPLICATION.