Syllabus For The Subject PRACTICAL VI - BIOTECHNOLOGY AND MICROBIOLOGY

UNIT - 01

1. PREPARATION AND REQUIREMENTS OF MICROBIOLOGY LABORATORY

2. MICROSCOPY THE OPERATIONAL USES OF LIGHT MICROSCOPE, PREPARATION, USE

AND CARE

3. STERILIZATION METHODS

4. STAINING - SIMPLE, DIFFERENTIAL (GRAM STAINING AND ACID FAST STAINING),

CAPSULAR, FUNGAL STAINING (LACTO PHENOL COTTON STAINING)

5. MEDIA PREPARATION - NUTRIENT BROTH, NUTRIENT AGAR, MS MEDIUM

6. ELECTROPHORESIS, CENTRIFUGE, COLORIMETER, HOT AIR OVEN, AUTOCLAVE,

LAMINAR AIR FLOW PH METER- DEMONSTRATION ONLY

7. GANONG`S PHOTOMETER, GANONG`S RESPIROSCOPE, OSMOSCOPE, WILMOT`S

BUBBLER.

8. STUDY LOCAL VEGETATION

9. STUDY OF MORPHOLOGICAL AND ANATOMICAL ADAPTATIONS OF PLANTS IN

DIFFERENT ECOLOGICAL CONDITIONS.

MAX : 75 MARKS

PRACTICAL : 65 MARKS

RECORD : 10 MARKS

QUESTION PAPER

1. MAKE A SUITABLE STAINED PREPARATION FOR THE GIVEN SPECIMEN "A". WRITE THE

PROCEDURE AND PRINCIPLE. DRAW LABELED SKETCHES. LEAVE THE SLIDE FOR

EVALUATION. (1X14=14)

2. ESTIMATE THE BIOCHEMICAL CONTENT OF THE PROVIDED PLANT SAMPLE "B". WRITE

THE PROCEDURE AND LEAVE THE RESULTS FOR EVALUATION. (1X12=12)

3. PREPARE THE MICROBIAL/BIOTECHNOLOGICAL NUTRIENT MEDIA USING THE

INGREDIENTS PROVIDED TO YOU IN "C" (1X7=7)

4. PERFORM THE PHYSIOLOGICAL EXPERIMENT PROVIDED TO YOU IN "D". WRITE THE

AIM, REQUIREMENT, PROCEDURE, RESULT AND LEAVE THE SET UP FOR THE

EVALUATION. (1X12=12)

5. MAKE A SUITABLE MICROSCOPIC PREPARATION OF THE SPECIMEN "E" AND IDENTIFY

THE HABITAT OF THE PLANT GIVING REASONS WITH SUITABLE DIAGRAMS. (1X10=10)

6. IDENTIFY THE GIVEN SPOTTERS "F, G, H, I & J". GIVE REASONS. DRAW DIAGRAMS.

(5X2=10)

SCHEME OF EVALUATION:

Q.NO.1.

PROCEDURE - 5 MARKS

PRINCIPLE - 5 MARKS

RESULT - 1 MARKS

SLIDE - 3 MARKS

DIAGRAM - 2 MARKS

Vinayaka Missions University,Directorate of Distance Education

Salem India

BACHELOR OF SCIENCE IN BOTANY

3 Yr.

PRACTICAL VI - BIOTECHNOLOGY AND MICROBIOLOGY(1030336)

TOTAL = 16 MARKS

Q.NO.2.

REQUIREMENTS - 2 MARKS

PROCEDURE - 5 MARKS

FORMULA - 2 MARKS

RESULT - 3 MARKS

TOTAL = 12 MARKS

Q.NO.3.

REQUIREMENT - 2 MARKS

PROCEDURE - 3 MARKS

RESULT - 2 MARKS

TOTAL = 7 MARKS

Q.NO.4.

AIM - 2 MARKS

REQUIREMENT - 2 MARKS

PROCEDURE - 4 MARKS

RESULT - 2 MARKS

SETUP - 2 MARKS

TOTAL = 12 MARKS

Q.NO.5.

IDENTIFICATION - 2 MARKS

REASON - 3 MARKS

SKETCH - 2 MARKS

SLIDE - 3 MARKS

TOTAL = 10 MARKS

Q.NO.6.

IDENTIFICATION - 1 MARK

REASON & SKETCHES - 1 MARK

TOTAL 5 X 2 = 10 MARKS