Syllabus For The Subject GENERAL MICROBIOLOGY AND IMMUNOLOGY

UNIT - 01

HISTORY OF MICROBIOLOGY - INTRODUCTION - SPONTANEOUS GENERATION - THE

GERM THEORY OF DISEASE OF DISEASE - CONTRIBUTIONS OF LOUIS PASTEUR -

ROBERT KOCH AND OTHERS TO THE FIELD OF MICROBIOLOGY.

UNIT - 02

MICROSCOPY - INTRODUCTION - DEFINITION - CLASSIFICATION - TYPES - LIGHT

MICROSCOPE - COMPOUND MICROSCOPE - PHASE CONTRAST MICROSCOPE -

FLUORESCENT MICROSCOPE - ELECTRON MICROSCOPE.

UNIT - 03

STERILIZATION AND DISINFECTION - INTRODUCTION - DEFINITION - TYPES OF METHODS

- PHYSICAL AND CHEMICAL METHODS - PHYSICAL METHODS - SUNLIGHT - DRYING - DRY

HEAT - MOIST HEAT - FILTRATION - RADIATION - ULTRASONIC AND SONIC VIBRATIONS -

DISINFECTION - ALCOHOL - ALDEHYDES - DYES - HALOGENS - PHENOLS - SURFACE

ACTIVE AGENTS - METALLIC SALTS - GASES.

UNIT - 04

CULTURE MEDIA - INTRODUCTION - DEFINITION - CLASSIFICATION - PREPARATION OF

CULTURE MEDIA - AEROBIC AND ANAEROBIC CULTURE METHODS - TYPES OF MEDIA -

SOLID MEDIA - LIQUID MEDIA - SEMISOLID MEDIA - SIMPLE MEDIA - COMPLEX MEDIA -

SYNTHETIC OR DEFINED MEDIA - ENRICHED MEDIA - ENRICHMENT MEDIA - SELECTIVE

MEDIA - INDICATOR MEDIA - DIFFERENTIAL MEDIA - SUGAR MEDIA - TRANSPORT MEDIA -

ANAEROBIC MEDIA - BACTERIAL COLONY MORPHOLOGY.

UNIT - 05

STAINING - INTRODUCTION - DEFINITION - CLASSIFICATION - PREPARATION OF SMEARS

- GRAM`S STAINING - ACID FAST STAINING - CAPSULAR STAINING - SPORE STAINING -

FLAGELLA STAINING.

UNIT - 06

ANTIBIOTICS - INTRODUCTION - CLASSIFICATION - ANTIBIOTICS AND THEIR MODE OF

ACTION - INHIBITION OF CELL-WALL SYNTHESIS - THE DAMAGE TO CYTOPLASMIC

MEMBRANE - INHIBITION OF SPECIFIC ENZYME SYSTEM - SULFONAMIDE - ANTIBIOTIC

SENSITIVITY TEST.

UNIT - 07

HISTORY OF IMMUNOLOGY - INTRODUCTION - DEFINITION - STRUCTURES -

COMPOSITION AND FUNCTION OF CELLS AND ORGANS INVOLVED IN IMMUNE SYSTEM -

HOST PARASITE RELATIONSHIP.

UNIT - 08

MICROBIAL INFECTION - INTRODUCTION - DEFINITION - VIRULENCE AND HOST

RESISTANCE - IMMUNE RESPONSES - INNATE IMMUNITY - ACQUIRED IMMUNITY -

DEFINITION - FUNCTION.

Vinayaka Missions University,Directorate of Distance Education

Salem India

BACHELOR OF SCIENCE IN BIO TECHNOLOGY

2 Yr.

GENERAL MICROBIOLOGY AND IMMUNOLOGY(1031012)

UNIT - 09

ANTIGENS - INTRODUCTION - DEFINITION - STRUCTURE AND PROPERTIES - TYPES - ISO

AND ALLO - HAPTENS - ADJUVANT - ANTIGEN SPECIFICITY.

UNIT - 10

ANTIBODY - INTRODUCTION - DEFINITION - CLASSIFICATION - STRUCTURE -

HETEROGENEITY - TYPES AND PROPERTIES OF ANTIBODIES.

UNIT - 11

COMPLEMENT - INTRODUCTION - DEFINITION - STRUCTURE - COMPONENTS -

PROPERTIES AND FUNCTIONS OF COMPLEMENT COMPONENTS - COMPLEMENT

PATHWAY AND BIOLOGICAL CONSEQUENCES OF COMPLEMENT ACTIVATION.

UNIT - 12

IN VITRO METHODS - AGGLUTINATION - PRECIPITATION - COMPLEMENT FIXATION -

IMMUNO FLUORESCENCE - ELISA - RADIO IMMUNE ASSAYS.