Syllabus For The Subject PRACTICAL III (BSC-BIOTECH)

UNIT - 01

1.IDENTIFICATION OF DRUMSTICK CHROMOSOMES IN HUMAN BLOOD.

2.ESTIMATION OF DNA IN A GIVEN SAMPLE

3.ESTIMATION OF RNA IN A GIVEN SAMPLE

4.PCR TECHNIQUE - DNA ANALYSIS - DEMONSTRATION.

5.BLOOD GROUP DETERMINATION BY SLIDE AGGLUTINATION; RH FACTOR

DETERMINATION BY

6.AGGLUTINATION REACTION.

7.TOTAL COUNT OF BLOOD CELLS AND DIFFERENTIAL COUNTS.

8.TOTAL HEMOGLOBIN DETERMINATION.

9.SEPARATION OF LYMPHOCYTES.

10.AG-AB REACTION (MODEL) - RAISING ANTIBODIES OF AG INJECTION (MODEL) -

BOOSTER

11.INJECTIONS- COLLECTION OF ANTISERUM - IMMUNODIFFUSION- RING TEST.

12.ELISA TECHNIQUE (DEMONSTRATION).

13.IMMUNOELECTROPHORESIS- WESTERN BLOTTING (DEMONSTRATION).

14.WIDAL TEST FOR TYPHOID FEVER.

15.CHART - IMMUNOGLOBULIN (G, A, M, D AND E) AND AIDS.