Syllabus For The Subject BIOPHYSICS AND CRYSTALLO

 

UNIT - 01

INTRODUCTION TO PROTEIN STRUCTURE - PHYSICAL AND CHEMICAL PROPERTIES OF

AMINO ACIDS AND POLYPEPTIDES - THEORETICAL AND EXPERIMENTAL METHODS FOR

DETERMINATION OF SIZE OF PROTEINS - PHYSICAL NATURE OF NON COVALENT

INTERACTIONS

UNIT - 02

CONFORMATIONAL PROPERTIES OF PROTEINS - RAMACHANDARN PLOT - SECONDARY -

SUPER SECONDARY - TERTIARY AND QUATERNARY STRUCTURE OF PROTEINS

UNIT - 03

PROTEIN STRUCTURE MODELING - HOMOLOGY MODELING - THREADING - FOLD

RECOGNITION - VECTOR BASED METHOD - NEURAL NETWORK

UNIT - 04

MODEL REFINEMENT AND VALIDATION - FUNCTIONAL CLASSIFICATION OF PROTEINS -

CELL SURFACE RECEPTORS - GPCR`S - KINASES - CHANNEL PROTEINS - UBIQUITIN

UNIT - 05

BIOPHYSICAL TECHNIQUES - PRINCIPLES - PROCESS AND APPLICATIONS OF THIN

LAYER CHROMATOGRAPHY - COLUMN CHROMATOGRAPHY ( ION EXCHANGE AND

AFFINITY ONLY) - HPLC

UNIT - 06

UNIDIRECTIONAL AND 2 D ELECTROPHORESIS

UNIT - 07

UV VISIBLE SPECTROSCOPY - NMR AND MALDI - TOF

UNIT - 08

X-RAY CRYSTALLOGRAPHY OF SMALL MOLECULES - X-RAY GENERATION - ITS

APPLICATION - UNIT CELL AND X-RAY ANOMALOUS SCATTERING

UNIT - 09

LATTICES - BRAGG`S LAW - ATOMIC SCATTERING FACTOR AND STRUCTURE FACTOR -

PHASE PROBLEM - INTENSITY DATA COLLECTION AND REDUCTION - DIRECT METHOD

OF SOLVING A SMALL MOLECULE - REFINEMENT OF CRYSTAL STRUCTURE -

HYDROGEN BONDING

UNIT - 10

X-RAY - CRYSTALLOGRAPHY OF MACROMOLECULES - ISOLATION AND PURIFICATION OF

PROTEIN

(CHROMATOGRAPHY, ELECTROPHORESIS) - CRYSTALLIZATION (SITTING AND HANGING

DROP METHOD)

UNIT - 11

PROTEIN STRUCTURE DETERMINATION-MOLECULAR REPLACEMENT TECHNIQUE -

MULTIPLE ISOMORPHISM REPLACEMENT METHOD - SYNCHROTRON RADIATION AND ITS

Vinayaka Missions University,Directorate of Distance Education

Salem India

BACHELOR OF SCIENCE IN BIO TECHNOLOGY

3 Yr.

BIOPHYSICS AND CRYSTALLOGRAPHY(1030807)

USES

UNIT - 12

PROTEIN STRUCTURE DETERMINATION - MULTI WAVELENGTH ANOMALOUS -

DIFFRACTION METHOD - CALCULATION OF ELECTRON DENSITY MAP - INTERPRETATION

OF ELECTRON DENSITY MAP - REFINEMENT OF THE STRUCTURE - STRUCTURE

VALIDATION METHODS