Syllabus For The Subject PRACTICAL V - CELL AND MOLECULAR BIOLOGY, MOLECULA

UNIT - 01

1. ISOLATION OF MICROBES (FUNGI, BACTERIA AND ACTINOMYCETES) FROM SOIL -

SERIAL DILUTION TECHNIQUES

2. SCREENING OF MICROBES FOR ENZYMES (AMYLASE) PRODUCTION PLATE AND

ENZYME ASSAY

METHOD

3. ISOLATION / EXTRACTION OF INTRACELLULAR AND EXTRA CELLULAR ENZYMES

4. CONSTRUCTION OF PURIFICATION TABLE FOR ANY ONE ENZYME

5. MOLECULAR WEIGHT DETERMINATION (SDS - PAGE)

6. NATIVE PAGE AND ELUTION OF ENZYME FROM THE GEL

NUCLEIC ACID LAB

7. ISOLATION OF DNA FROM MICROBIAL AND ANIMAL CELLS

8. ISOLATION OF RNA FROM BAKERS YEAST

9. ISOLATION OF PLASMID FROM BACTERIUM

10. RESTRICTION, DIGESTION AND LIGATION

11. PCR AMPLIFICATION

IMMUNOLOGY LAB

12. METHODS OF BLEEDING

13. COUNTING OF BLOOD CELLS- HAEMOCYTOMETRE COUNT

14. PREPARATION OF SERUM FROM BLOOD

15. BLOOD GROUPING- HAEMOAGGLUTINATION

16. ELISA

PHARMACOLOGY LAB

17. SCREENING OF MICROBES FOR ANTIBIOTIC PRODUCTION

18. ANTIBIOTIC BIOASSAY(KIRBY BAUER METHOD)

19. EXTRACTION OF ANY ONE PHARMACEUTICAL COMPOUND FROM MEDICINAL PLANTS

(SOXLET -

EXTRACTION METHOD)

20. TISSUE CULTURE TECHNIQUE FOR SECONDARY METABOLITES PRODUCTION

21. STRUCTURE PREDICTION OF PHARMACEUTICAL COMPOUND BY NMR.

UNIT - 02

1. PURE CULTURE TECHNIQUES

2. PREPARING COMPETENT CULTURE

3. AGAROSE GEL ELECTROPHORESIS

4. SDS-PAGE

5. BLOTTING TECHNIQUES

6. PCR

UNIT - 03

1. MICROBIAL GENOMIC DNA ISOLATION

2. MICROBIAL PLASMID ISOLATION

3. PLANT GENOMIC DNA ISOLATION

Vinayaka Missions University,Directorate of Distance Education

Salem India

BACHELOR OF SCIENCE IN BIO TECHNOLOGY

3 Yr.

PRACTICAL V - CELL AND MOLECULAR BIOLOGY, MOLECULAR

TECHNIQUES(1030810)

4. ANIMAL GENOMIC DNA ISOLATION

5. RESTRICTION MAPPING WITH LAMDA DNA

6. LIGATION