Syllabus For The Subject PRACTICAL IV

UNIT - 01

1.PREPARATION OF NON-SELECTIVE AND SELECTIVE MEDIA - ENUMERATION OF

BACTERIA - FUNGI AND ACTINOMYCETES [PLATE COUNT] FROM SOIL AND WATER.

2.ISOLATION OF PATHOGENIC MICROORGANISMS FROM INFECTED TISSUE.

3.OBSERVATION OF MORPHOLOGICAL CHARACTERISTICS OF MOULD FUNGI.

4.PURIFICATION OF MIXED CULTURES.

5.ANTIBIOTIC SENSITIVITY TEST.

6.OBSERVATION OF MOTILITY OF BACTERIA [HANGING DROP TECHNIQUE.

7.STAINING METHODS - PREPARATION OF SMEARS FOR STAINS, SIMPLE STAINING,

NEGATIVE STAINING,GRAM STAINING.

8.TEST FOR COLIFORM BACTERIA.

9.BACTERIAL ENUMERATION OF MICROORGANISMS FROM DIFFERENT TYPES OF

INFECTED HOSPITAL SAMPLES - URINE, STOOL, PUS, BLOOD AND CEREBROSPINAL

FLUID.

10.SPOILAGE OF MILK BY MICROORGANISMS [METHYLENE BLUE TEST].

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